Novel peptide ligands identified using human genomic sequence data and state-of-the-art bioinformatic algorithms
Most GPCRs are important pharmaceutical drug targets. However, the endogenous ligands for many GPCRs have not been identified. Those GPCRs are referred as orphan GPCR. About half of the orphan GPCRs are predicted to be peptide receptors. To help identify endogenous ligand for orphan receptors, Qventas is now offering orphan peptide ligand libraries to the GPCR research community.
The exploding of genome sequence data provides a rich resource for novel ligand peptide discovery. Using a proprietary bioinformatics algorithm, we have identified many potential novel GPCR peptide ligands in the human genomic sequence database. The core technology of this proprietary discovery process integrates together the following bioinformatics algorithms:
- Gene prediction: algorithms to identify new genes from genomic sequence data.
- Cellular localization: bioinformatics method to predict cellular localization of a protein.
- Signal peptide and transmembrane region: algorithm to identify signal peptides and hydrophobic transmembrane regions of a protein.
- Precursor protein processing: identification of potential processing cutting site.
- Motif and pattern analysis: database mining using characteristic motif in known ligand peptides.
- Comparative genomics: algorithm to identify ortholog/homolog across species (e.g. human vs. mouse)
Besides computational analysis, all the candidate genes are manually reviewed for quality control. As a positive control, we successfully identified almost all the literature documented ligand peptides using this proprietary algorithm.
Advantage of Qventas Technology
Peptide ligand plates offered by most vendors are dominated with well-characterized peptide ligands (paired with known GPCR). The chance for a known peptide ligand to match a novel GPCR is small based on previous discovery experiences in the GPCR research community. To maximize the chance of identifying ligand for an orphan GPCR, we are now offering orphan peptide ligand libraries to the GPCR research community. Our price is very competitive compared with other vendors which provide only known ligands.
The first plate (QVL-A) that we are offering includes 80 peptides in a 96 well plate, and additional plates will be offered in the near future.
Peptide Ligand Library Description
A) Library A (QVL-A) contains 80 peptides in a 96 well plate. 3 nanomoles of each peptide is dissolved in 30 microliters of PBS with 15 micrograms of BSA. Price is $1260/Library. This library can be used for at least 24 FLIPR screening assays.
B) Peptide sequence information in libraries. Because peptide sequence information in libraries are our very important intellectual property. We will only release these information to our customers under certain confidentiality agreement and with some additional cost. If you are interested in certain peptide information, the cost is $300 per peptide (including precursor protein sequence, and peptide sequence). If you are interested in obtaining all the peptide sequence information for library A, the price is reduced to $100 per peptide.